Protein expression analysis
Strain specific hB2M expression analysis in heterozygous B-hB2M/HLA-G (H/+) mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and heterozygous B-hB2M/HLA-G (H/+) mice, and analyzed by flow cytometry with species-specific anti-B2M antibody. Mouse B2M was detectable in wild-type mice and heterozygous B-hB2M/HLA-G (H/+). Human B2M was exclusively detectable in heterozygous B-hB2M/HLA-G (H/+) mice but not wild-type mice.
Strain specific HLA-G expression analysis in heterozygous B-hB2M/HLA-G (H/+) mice by flow cytometry. Splenocytes were collected from wild-type mice (+/+) and heterozygous B-hB2M/HLA-G mice (H/+) ,and analyzed by flow cytometry with species-specific anti-HLA-G antibody. Human HLA-G was exclusively detectable in heterozygous B-hB2M/HLA-G (H/+) but not in wild-type mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hB2M/HLA-G mice] (Cat# 111339) was purchased from Biocytogen.
