Targeting strategy
Gene targeting strategy for B-hCTLA4/hTNFR2 mice. The exon 2 of mouse Ctla4 gene that encodes the extracellular domain was replaced by human CTLA4 exon 2 in B-hCTLA4/hTNFR2 mice. The exons 2~6 of mouse Tnfr2 gene that encode the extracellular domain was replaced by human TNFR2 exons 2~6 in B-hCTLA4/hTNFR2 mice.
Protein expression analysis
Strain specific hCTLA4 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD152 (CTLA4) antibodies. Mouse CTLA4 was detectable in wild-type mice. Human CTLA4 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
Strain specific hTNFR2 expression analysis in homozygous B-hCTLA4/hTNFR2 mice by flow cytometry. Spleen was collected from wild-type mice (+/+) and homozygous B-hCTLA4/hTNFR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-TNFR2 antibodies. Mouse TNFR2 was detectable in wild-type mice. Human TNFR2 was exclusively detectable in homozygous B-hCTLA4/hTNFR2 mice but not in wild-type mice.
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hCTLA4/hTNFR2 mice] (Cat# 111843) was purchased from Biocytogen.
