Strain specific analysis of IL6 expression in B-NDG mice and B-NDG hIL6 mice by ELISA. Serum was collected from B-NDG mice (+/+) and homozygous B-NDG hIL6 mice (H/H) stimulated with LPS, and analyzed by ELISA with anti-IL6 antibodies. Mouse IL6 was detectable in B-NDG mice. Human IL6 was exclusively detectable in homozygous B-NDG hIL6 mice but not in B-NDG mice.

B-NDG hIL6 mice are well suited for human monocytes reconstitution. Human CD34+ HSCs (0.15 M) were intravenous implanted into homozygous B-NDG hIL6 and B-NDG mice (female, 6 week-old, n=15). All mice were treated with 1.6 Gy-irradiation. Flow cytometry analysis were performed with PBMCs from mice engrafted with human CD34+ HSCs. B-NDG hIL6 mice show higher percentages of human myeloid cells and monocytes compared with B-NDG mice.