Homozygous generally embryonic lethal, behave abnormal blood vessel development. In this strain, iCre-ERT2 recombinase expression is under the control of Cdh5 promoter. An iCre-ERT2 sequence cassette was placed between the coding sequence of exon 2 and 5’UTR of the Cdh5 gene, and the expression of endogenous Cdh5 gene in mice was disrupted. When crossed with a strain containing a loxP-site flanked sequence of interest, and induced with Tamoxifen, Cre-mediated recombination will result in deletion of the floxed sequence in the offspring.

Immunofluorescent images of tdTomato and CD31 in the main organs of double-positive mice. CD31 is endothelial marker. Scale bar, 25 µm.