C57BL/6-Col10a1tm1(icre)Bcgen/Bcgen • 110122
Homozygous mice for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities
Gel image of PCR products. PCR products are separated by gel electrophoresis on a 2% agarose gel. PC: samples used for PCR were collected from heterozygous mice; WT: samples used for PCR were collected from wild type mice; H2O: blank control.
Col10-Cre-marked cells, which excluded periosesteal cells, gave rise to ~58%of all Lepr+ BMSCs.
(C) Confocal imaging of femur sections from Col10cre; R26tdTomato mice at P3 showed that Col10-Cre (Col10-Cre was purchased from Biocytogen Co. Ltd., Beijing, China) marked cells included chondrocytes, but not periosesteal cells. Asterisks indicated the periosesteum. (n=3 mice from 3 independent experiments)
(D and E) Confocal imaging (D) and flow cytometry (E) showed that Col10-Cre marked cells gave rise to most of the Lepr+ BMSCs at 6 months of age. (n = 3 mice from 3 independent experiments)
