Expression
Expressed gene
iCre, improved crerecombinase, bacteriophage P1
Site of expression
endothelial cells, centralnervous system (CNS) blood vessels
Gene editing strategy
An F2A-iCre sequence cassette was placed between the coding sequence of exon 23 and 3'UTR of the Pdgfrb gene. This strain was maintained on a C57BL/6 genetic background.
Phenotype analysis
(B) Quantification of the number of CD31+ capillaries expressing claudin-5 or ZO-1 and the MFI of claudin-5 or ZO-1 in CD31+ capillaries showed reduction of claudin-5 in capillaries of cortex from Cd146−/− mice compared with those from Cd146+/+ mice at P5 and 4–6 wk. (C) Brain sections from cortex of mice at 4–6 wk were costained for CD31 (green) and PDGFRβ (red) and analyzed by LSFM after being optically cleared by using organic solvents. Representative MIPs of 40 virtual single slices from Cd146+/+ and Cd146−/− mice are shown. (Scale bar: 50 μm.)(source: Chen J et al.,2017)
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-Pdgfrb-iCre mice] (Cat# 110129) was purchased from Biocytogen.
